Know the enemy: Contam - Revision history

QuintinB at 13:29, 7 June 2020

2020-06-07T13:29:04Z

QuintinB: /* 4. Leaving spawn jars for too long before transferring them */

2020-06-07T13:23:19Z

4. Leaving spawn jars for too long before transferring them

QuintinB: Created page with "Here are some of the ways contam kicked my ass this year: ===1. Too much liquid introduced from an LC=== ===2. Too much moisture on the grains=== ===3. Too much moisture in..."

2020-06-07T13:23:04Z

Created page with "Here are some of the ways contam kicked my ass this year: ===1. Too much liquid introduced from an LC=== ===2. Too much moisture on the grains=== ===3. Too much moisture in..."

New page

Here are some of the ways contam kicked my ass this year:

===1. Too much liquid introduced from an LC===

===2. Too much moisture on the grains===

===3. Too much moisture in the substrate===

===4. Leaving spawn jars for too long before transferring them===
This starves the organism and cuts bacteria and trich some slack.

===5. Leaving petris in the fridge for long before using them===
Especially when I keep opening the bag to remove 1/2 petris at at time or carrying the bag between the SAB and the fridge.

← Older revision		Revision as of 13:29, 7 June 2020
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	===5. Leaving petris in the fridge for long before using them===		===5. Leaving petris in the fridge for long before using them===
	Especially when I keep opening the bag to remove 1/2 petris at at time or carrying the bag between the SAB and the fridge.		Especially when I keep opening the bag to remove 1/2 petris at at time or carrying the bag between the SAB and the fridge.
		+
		+	===6. Innoculating a sterile LC with too little mycelium===
		+	Innoculating an LC with a largish agar wedge ensures the organism recovers quickly. I find more contamination when doing innoculations with biopsies or tiny agar wedges. Especially with biopsies where I think tiny contams on the mycelium are then on a more equal footing with your own organism.

@@ Line 8: / Line 8: @@
 ===4. Leaving spawn jars for too long before transferring them===
-This starves the organism and cuts bacteria and trich some slack.
+This starves the organism and cuts contam some slack.
 ===5. Leaving petris in the fridge for long before using them===
 Especially when I keep opening the bag to remove 1/2 petris at at time or carrying the bag between the SAB and the fridge.